Background: Although various researches have been conducted to improve therapeutic strategies in resolving spinal cord injuries, robust clinical treatment is not yet available. Developing a standard animal model is essential before treatment.
Objectives: The present st More
Background: Although various researches have been conducted to improve therapeutic strategies in resolving spinal cord injuries, robust clinical treatment is not yet available. Developing a standard animal model is essential before treatment.
Objectives: The present study was performed to introduce a simple, applicable, and minimally invasive approach for access to epidural space in cat.
Methods: We used per-cutaneous approach from lumbosacral junction for stainless steel cannula insertion to the epidural space. CT-scan, conventional Magnetic Resonance Imaging, tractography, and behavioral evaluation were used to assess the correct position of cannula and neurological condition of the patient.
Results: MRI results showed no significant change in signal intensity index of neural structures under lumbosacral junction. These observations were further supported by tractography, and also behavioral examination during study.
Conclusions: We found that per-cutaneous approach from lumbosacral junction is a simple and applicable approach which has no side effects and artifact formation in MRI evaluation.
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Sepsis is a complex and dynamic syndrome and it is a medical and economic challenge. To learn more about pathophysiology of this syndrome, animal models have been introduced. Poly microbial sepsis induced by cecal ligation and puncture is the gold standard model of this More
Sepsis is a complex and dynamic syndrome and it is a medical and economic challenge. To learn more about pathophysiology of this syndrome, animal models have been introduced. Poly microbial sepsis induced by cecal ligation and puncture is the gold standard model of this condition. The purpose of this study was introducing a new method of septic peritonitis with laparoscopic assisted cecal ligation and cauterization (CLC) in rabbit model. This study included two groups of adult male New Zealand white rabbits:
Control group (4rabbits): exploratory laparoscopy was performed and the cecum was grasped from the distal of ileocecal valve using an atraumatic forceps and pulled out from the trocar entry site. CLC group (4 rabbits): the cecum was ligated and two sites of cecum were cauterized from antimesenteric to mesenteric surface of cecum. Before and during 24 hours after the operation, heart rate, rectal temperature and respiratory rate of rabbits were monitored. Ultrasonography, CBC, peritoneal fluid analysis and bacterial culture was checked 24 hours after the surgery. Statistical analysis of the data in CLC group rabbits showed a significant increase in heart rate 6 and 18 hours after surgery (tachycardia) and increase in respiratory rate from 6 to 24 hours after surgery (tachypnea). In addition, a significant decrease in glucose of serum was observed. Bacterial culture was positive and peritoneal analysis of all rabbits in CLC group indicated the presence of bacteria and infection.
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